Z-WEHD-FMK NLT 98%

Z-WEHD-FMK is a potent, cell-permeable and irreversible caspase-1/5 inhibitor. Z-WEHD-FMK also exhibits a robust inhibitory effect on cathepsin B activity (IC₅₀=6 μM). Z-WEHD-FMK can be used to investigate cells for evidence of apoptosis^[1][2][4]. IC50 & Target: IC50: caspase-1/5; 6 μM (cathepsin B)^[1][2] In Vitro: Z-WEHD-FMK (80 μM; 9 hours) elicits a near-complete blockage of C. trachomatis-induced cleavage of golgin-84 and increases GM130 expression in cells^[1].
Z-WEHD-FMK (30 min before being exposed to E. piscicida) effectively inhibits 0909I E. piscicida induced ZF4 cells cytotoxicity and pyroptotic morphology. And in addition, it also inhibits the cytotoxicity induced by cytosolic LPS delivery^[2].
Z-WEHD-FMK (20 μM;18-24 hours following Cr³⁺,Ni²⁺, and Co²⁺) significantly induces a decrease of 76% to 86% in IL-1β release with 200 to 400 ppm Cr³⁺, it also induces a decrease of 35% to 45% with 48 ppm Ni²⁺ or higher, Finally, this caspase-1 inhibitor induced a decrease with 6 ppm Co²⁺, down to a level below the detection threshold, and a decrease of 40% to 48% with 12 to 24 ppm Co²⁺ in bone marrow-derived macrophages (BMDM)^[3].